Nucleoside reverse transcriptase inhibitors
Nucleoside reverse transcriptase inhibitors (NRTIs) are analogues of the natural RNA and DNA nucleotides. Zidovudine (AZT) e.g. is a thymidine analogue. This drug is phosphorylated three times, like its natural analogue and together they compete for the reverse transcriptase. The triphosphate drug inhibits the reverse transcriptase. However when transcription still occurs, the triphosphate drug is built in the DNA. The incorporation of nucleoside analogues aborts DNA synthesis and destabilizes the viral double DNA strand. Zidovudine was the first antiretroviral agent. In high doses it can lead to
severe myelotoxicity, but in normal doses it causes only some initial gastrointestinal complaints. Zidovudine is a favourable NRTI because it lacks neurotoxicity and good CNS penetration.
Herpes-simplex-virus (HSV) is NOT sensitive to zidovudine (AZT, a reverse transcriptase inhibitor). The reason is that HSV uses its own DNA polymerase for replication.
Extra info: Zidovudine inhibits the viral reverse transcriptase. The HSV virus has no reverse transcriptase, because it is a DNA virus.
Pregnancy is a contra-indication for the use of zidovudine.
Extra info: HIV-positive pregnant women can use zidovudine in order to prevent viral transfer from mother to child.
Zidovudine is able to pass the blood-brain-barrier.
Drugs of choice in HIV infections are two NRTIs and a protease inhibitor (triple therapy).
Extra info: This cocktail has led to the long-term survival of HIV positive patients.